Gastrin stimulates the formation of a p60Src/p125FAK complex upstream of the phosphatidylinositol 3‐kinase signaling pathway

FEBS Letters

Seva, Catherine¹  Pradayrol, Lucien¹  Vaysse, Nicole¹  Daulhac, Laurence¹  Kowalski-Chauvel, Aline¹ 

[1] INSERM U.151, Groupe de Recherche de Biologie et Pathologie Digestive, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse, France

关键词: Gastrin;    G protein-coupled receptor;    Phosphatidylinositol 3-kinase;    Src-family kinases;    Focal adhesion kinase;    Cellular proliferation;   

DOI  :  10.1016/S0014-5793(99)00129-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular events whereby gastrin occupancy of G/CCK_B receptors leads to phosphatidylinositol (PI) 3-kinase activation have been examined. We report here that this peptide promotes the association between two non-receptor tyrosine kinases, p60^Src and p125^FAK, and elicits a parallel increase in tyrosine phosphorylation and activity of both kinases. Gastrin-induced PI 3-kinase activity was coprecipitated with p60^Src and p125^FAK and was inhibited by herbimycin A, the selective Src inhibitor PP-2 or cytochalasin D, which disrupts the actin cytoskeleton and prevents p125^FAK activity. These results indicate, for the first time, that a p60^Src/p125^FAK complex acts upstream of the gastrin-stimulated PI 3-kinase pathway.

【 授权许可】

Unknown