Cellular localization and evolution of prolactin receptor mRNA in ovine endometrium during pregnancy

FEBS Letters

Djiane, Jean¹  Charlier, Madia¹  Pessemesse, Laurence¹  Cassy, Sandrine¹  Guillomot, Michel¹ 

[1] Laboratoire de Biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas 78352, France

关键词: Prolactin receptor;    Endometrium;    Ovine species;    In situ hybridization;   

DOI  :  10.1016/S0014-5793(99)00052-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we have investigated the expression of the prolactin receptor gene in ovine endometrium during oestrus cycle and pregnancy. Using reverse transcription-PCR analysis, we provided evidence that the prolactin receptor gene is specifically transcribed in this tissue. As shown by Northern blot analysis, the level of the prolactin receptor transcripts increased dramatically during late pregnancy. In situ hybridization experiments revealed that prolactin receptor mRNA was specifically expressed in the glandular compartment and confirmed the dramatic increase of its expression that occurs at the end of pregnancy. Taken together, these findings are consistent with a putative role of prolactin and/or related molecules in the regulation of the proliferation of the glandular compartment and/or in the control of the secretory activity of the endometrium.

【 授权许可】

Unknown