Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci and Chlamydia pneumoniae

FEBS Letters

Ashley, R.H.¹  Wyllie, S.³  Longbottom, D.³  Herring, A.J.² 

[1] Membrane Biology Group, Dept of Biomedical Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK;The Public Health Laboratory, Kingsdown, Bristol BS2 8EL, UK;Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik, Midlothian EH26 OPZ, UK

关键词: Chlamydia;    Major outer membrane protein;    Planar lipid bilayer;    Porin;    Recombinant protein;   

DOI  :  10.1016/S0014-5793(99)00121-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently demonstrated that the major outer membrane protein of Chlamydia psittaci, the primary vaccine candidate for combating chlamydial infections, functions as a porin-like ion channel. In this study, we have cloned, expressed and functionally reconstituted recombinant major outer membrane proteins from C. psittaci and Chlamydia pneumoniae and analysed them at the single channel level. Both form porin-like ion channels that are functionally similar to those formed by native C. psittaci major outer membrane protein. Also, like the native channels, recombinant C. psittaci channels are modified by a native major outer membrane protein-specific monoclonal antibody. This is the first time that native function has been demonstrated for recombinant chlamydial major outer membrane proteins. Future bilayer reconstitution will provide a strategy for detailed structure/function studies of this new subclass of bacterial porins and the work also has important implications for successful protein refolding and the development of improved subunit vaccines.

【 授权许可】

Unknown