The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor

FEBS Letters

Weber, Thomas¹  Dameron, Charles T.²  Harrison, Mark D.²  Solioz, Marc¹  Cobine, Paul²  Wickramasinghe, Wasantha A.² 

[1] Department of Clinical Pharmacology, University of Berne, 3010 Berne, Switzerland;National Research Centre for Environmental Toxicology, University of Queensland, 39 Kessels Road, Coopers Plains, Queensland 4108, Australia

关键词: Copper chaperone;    Enterococcus hirae;    Metalloregulation;    Metal transfer;    Repressor;   

DOI  :  10.1016/S0014-5793(99)00091-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of the cop operon which effects copper homeostasis in Enterococcus hirae is controlled by the copper responsive repressor CopY. Purified Zn(II)CopY binds to a synthetic cop promoter fragment in vitro. Here we show that the 8 kDa protein CopZ acts as a copper chaperone by specifically delivering copper(I) to Zn(II)CopY and releasing CopY from the DNA. As shown by gel filtration and luminescence spectroscopy, two copper(I) are thereby quantitatively transferred from Cu(I)CopZ to Zn(II)CopY, with displacement of the zinc(II) and transfer of copper from a non-luminescent, exposed, binding site in CopZ to a luminescent, solvent shielded, binding site in CopY.

【 授权许可】

Unknown