Growth hormone induces insulin‐like growth factor‐I gene transcription by a synergistic action of STAT5 and HNF‐1α

FEBS Letters

Steenbergh, Paul H.¹  Sussenbach, John S.¹  Metón, Isidoro¹  Boot, Elmieke P.J.¹ 

[1] Laboratory for Physiological Chemistry, Utrecht University, P.O. Box 80042, 3508 TA Utrecht, The Netherlands

关键词: IGF-I;    Transcription activation;    GH;    STAT5;    HNF-1;    GAS;    γ-interferon-activated sequence;    GH;    growth hormone;    GH-R;    growth hormone receptor;    HNF;    hepatocyte nuclear factor;    IGF;    insulin-like growth factor;    JAK;    Janus kinase;    Luc;    luciferase;    MEM;    modified essential medium;    Spi;    serine protease inhibitor;    STAT;    signal transducer and activator of transcription;    TK;    thymidine kinase;   

DOI  :  10.1016/S0014-5793(99)00064-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Salmon insulin-like growth factor-I (sIGF-I) expression is, as in mammals, induced by growth hormone (GH). To elucidate the mechanism by which GH stimulates the transcription of the IGF-I gene, we transiently transfected Hep3B cells expressing the rat GH receptor with a sIGF-I promoter-luciferase reporter construct. Activation of the construct by GH added to the medium of the transfected cells was observed when two specific transcription factors, STAT5 and HNF-1α, were simultaneously overexpressed in these cells. This finding demonstrates for the first time a GH-dependent activation of an IGF-I promoter construct in an immortalized laboratory cell line.

【 授权许可】

Unknown