FEBS Letters | |
Growth hormone induces insulin‐like growth factor‐I gene transcription by a synergistic action of STAT5 and HNF‐1α | |
Steenbergh, Paul H.1  Sussenbach, John S.1  Metón, Isidoro1  Boot, Elmieke P.J.1  | |
[1] Laboratory for Physiological Chemistry, Utrecht University, P.O. Box 80042, 3508 TA Utrecht, The Netherlands | |
关键词: IGF-I; Transcription activation; GH; STAT5; HNF-1; GAS; γ-interferon-activated sequence; GH; growth hormone; GH-R; growth hormone receptor; HNF; hepatocyte nuclear factor; IGF; insulin-like growth factor; JAK; Janus kinase; Luc; luciferase; MEM; modified essential medium; Spi; serine protease inhibitor; STAT; signal transducer and activator of transcription; TK; thymidine kinase; | |
DOI : 10.1016/S0014-5793(99)00064-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Salmon insulin-like growth factor-I (sIGF-I) expression is, as in mammals, induced by growth hormone (GH). To elucidate the mechanism by which GH stimulates the transcription of the IGF-I gene, we transiently transfected Hep3B cells expressing the rat GH receptor with a sIGF-I promoter-luciferase reporter construct. Activation of the construct by GH added to the medium of the transfected cells was observed when two specific transcription factors, STAT5 and HNF-1α, were simultaneously overexpressed in these cells. This finding demonstrates for the first time a GH-dependent activation of an IGF-I promoter construct in an immortalized laboratory cell line.
【 授权许可】
Unknown
【 预 览 】
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