Localization of curved DNA and its association with nucleosome phasing in the promoter region of the human estrogen receptor α gene

FEBS Letters

Wada-Kiyama, Yuko¹  Onishi, Yoshiaki³  Trifonov, Edward N²  Kiyama, Ryoiti³  Kuwabara, Kentaro¹  Sakuma, Yasuo¹ 

[1] Department of Physiology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113, Japan;Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel;National Institute of Bioscience and Human Technology, Ministry of International Trade and Industry, 1-1 Higashi, Tsukuba, Ibaraki 305, Japan

关键词: DNA bend site;    Curvature;    Nucleosome mapping;   

DOI  :  10.1016/S0014-5793(99)00041-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We determined DNA bend sites in the promoter region of the human estrogen receptor (ER) gene by the circular permutation assay. A total of five sites (ERB−4 to −1, and ERB+1) mapped in the 3 kb region showed an average distance of 688 bp. Most of the sites were accompanied by short poly(dA)·poly(dT) tracts including the potential bend core sequence A₂N₈A₂N₈A₂ (A/A/A). Fine mapping of the ERB−2 site indicated that this A/A/A and the 20 bp immediate flanking sequence containing one half of the estrogen response element were the sites of DNA curvature. All of the experimentally mapped bend sites corresponded to the positions of DNA curvature as well as to nucleosomes predicted by computer analysis. In vitro nucleosome mapping at ERB−2 revealed that the bend center was located 10–30 bp from the experimental and predicted nucleosome dyad axes.

【 授权许可】

Unknown