Apocynin increases glutathione synthesis and activates AP‐1 in alveolar epithelial cells

FEBS Letters

Hiemstra, Pieter S²  MacNee, William¹  Stolk, Jan²  Lapperre, Thérèse S²  Antonicelli, Frank¹  Drost, Ellen M¹  Jimenez, Luis A¹  Rahman, Irfan¹ 

[1] Respiratory Medicine Unit, University of Edinburgh, Medical School, Teviot Place, Edinburgh EH8 9AG, UK;Department of Pulmonology, Leiden University Medical Centre, Leiden, The Netherlands

关键词: Glutathione;    Antioxidant;    Apocynin;    Activator protein-1;    A549;   

DOI  :  10.1016/S0014-5793(98)01723-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Apocynin (4-hydroxy-3-methoxy-acetophenone) is a potent intracellular inhibitor of superoxide anion production in neutrophils. In this study, we studied the effect of apocynin on the regulation of the antioxidant glutathione (GSH) and activation of the transcription factor AP-1 in human alveolar epithelial cells (A549). Apocynin enhanced intracellular GSH by increasing γ-glutamylcysteine synthetase activity in A549 cells. Apocynin also increased the expression of γ-GCS heavy subunit mRNA. This was associated with increased AP-1 DNA binding as measured by the electrophoretic mobility shift assay. These data indicate that apocynin displays antioxidant properties, in part, by increasing glutathione synthesis through activation of AP-1.

【 授权许可】

Unknown