Uni‐axial cyclic stretch induces c‐src activation and translocation in human endothelial cells via SA channel activation

FEBS Letters

Sokabe, Masahiro¹  Naruse, Keiji¹  Sai, Xaurui¹  Yokoyama, Nagao¹ 

[1] Department of Physiology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466, Japan

关键词: Ca2+;    Gd3+;    Calcineurin;    FK506;    ECV304;   

DOI  :  10.1016/S0014-5793(98)01528-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinase activity of c-src increased and peaked at 15 min after an application of uni-axial cyclic stretch in HUVECs followed by a translocation of c-src to Triton-insoluble fraction. Suppression of c-src by an antisense S-oligodeoxynucleotide inhibited the stretch-induced tyrosine phosphorylation and morphological changes. The stretch-induced increase in c-src activity was inhibited by FK506, a specific inhibitor for calcineurin, by Gd³⁺, a blocker for stretch activated channels, and by the extracellular Ca²⁺ depletion suggesting the involvement of SA channels. These results strongly suggest c-src plays an important role in the downstream of SA channel activation followed by the morphological changes.

【 授权许可】

Unknown