The CGRP receptor can couple via pertussis toxin sensitive and insensitive G proteins

FEBS Letters

Foord, Steven M¹  Fraser, Neil J¹  Brown, Susan¹  Brown, Jason¹  Main, Martin J¹ 

[1] Receptor Systems, GlaxoWellcome Research Ltd., Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK

关键词: Calcitonin-gene-related peptide receptor;    Receptor activity modifying protein 1;    G protein;    Potassium channel;   

DOI  :  10.1016/S0014-5793(98)01507-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Swiss 3T3 cell lines were constructed co-expressing receptor activity modifying protein (RAMP) 1 with the calcitonin receptor-like receptor (CRLR), and showed ¹²⁵I-calcitonin-gene-related peptide (CGRP) 1 binding indicative of a type I CGRP receptor. Application of CGRP1 led to an increase in cAMP, which in 2/5 cell lines was augmented following pertussis toxin (PTX) pre-treatment. In Xenopus oocytes, expression of RAMP1, which potentiates an endogenous CGRP receptor, led to constitutive activation of co-expressed GIRK potassium channels. This potassium current was increased following CGRP application or co-expression of CRLR, but decreased by PTX or co-expression of transducin. We conclude that the CGRP receptor can signal to both PTX sensitive and insensitive G proteins.

【 授权许可】

Unknown