Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action

FEBS Letters

Prestwich, R¹  Lange, L²  Collis, L³  Ziebell, M¹  Turley, E.A³  Hall, C² 

[1] Department of Medicinal Chemistry, University of Utah, 30 South 2000 East, Salt Lake City, UT 84112-5820, USA;Manitoba Institute of Cell Biology, 100 Olivia St., Winnipeg, Man. R3E 0V9, Canada;The Hospital for Sick Children, Division of Cardiovascular Research, 555 University Ave., Toronto, Ont. M5G 1X8, Canada

关键词: Hyaluronan;    RHAMM;    CD44;    Internalization;    Lamella;    Nucleus;   

DOI  :  10.1016/S0014-5793(98)01505-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Texas red-labeled hyaluronan (TR-HA) is rapidly taken up in a CD44 independent manner into ras-transformed 10T1/2 fibroblasts, where it accumulates in both cell ruffles/lamellae, the perinuclear area, and the nucleus. HA does not accumulate in the cell ruffles/lamellae of parental 10T1/2 cells. Addition of HA to ras-transformed cells promotes their random motility but has no effect on 10T1/2 cell motility. 10T1/2 cells can be modified to take up HA into cell ruffles by exposure to phorbol ester or direct microinjection of HA into cells. Both treatments significantly stimulate 10T1/2 cell motility.

【 授权许可】

Unknown