Identification of regions in which positive selection may operate in S‐RNase of Rosaceae: Implication for S‐allele‐specific recognition sites in S‐RNase

FEBS Letters

Endo, Toshinori¹  Yamaguchi-Kabata, Yumi³  Ishimizu, Takeshi²  Nakamura, Kazuo T.⁴  Sakiyama, Fumio²  Norioka, Shigemi² 

[1] Center for Information Biology, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan;Division of Protein Chemistry, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606-8502, Japan;School of Pharmaceutical Sciences, Showa University, Hatanodai, Shinagawa, Tokyo 142-8555, Japan

关键词: Self-incompatibility;    S-RNase;    Rosacea;    Positive selection;    Non-synonymous nucleotide substitution;    dS;    the number of synonymous substitutions;    dN;    the number of non-synonymous substitutions;    HV region;    hypervariable region;    PS region;    putative positively selected region;   

DOI  :  10.1016/S0014-5793(98)01470-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae, Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific recognition in the self-incompatible reaction, but how it functions in specific discrimination is not clear. Window analysis of the numbers of synonymous (d_S ) and non-synonymous (d_N ) substitutions in rosaceous S-RNases detected four regions with an excess of d_N over d_S in which positive selection may operate (PS regions). The topology of the secondary structure of the S-RNases predicted by the PHD method is very similar to that of fungal RNase Rh whose tertiary structure is known. When the sequences of S-RNases are aligned with the sequence of RNase Rh based on the predicted secondary structures, the four PS regions correspond to two surface sites on the tertiary structure of RNase Rh. These findings suggest that in S-RNases the PS regions also form two sites and are candidates for the recognition sites for S-allele-specific discrimination.

【 授权许可】

Unknown