Hyperthermophilic redox chemistry: a re‐evaluation

FEBS Letters

Landa, Ilse¹  Driessen, Martijn C.P.F¹  van den Bosch, Marieke¹  Hagedoorn, Peter L¹  Hagen, Wilfred R¹ 

[1] Wageningen University and Research Centre, Department of Biomolecular Sciences, Bioinorganic Chemistry Group, Dreijenlaan 3, NL-6703 HA Wageningen, The Netherlands

关键词: Rubredoxin;    Ferredoxin;    Reduction potential;    Hyperthermophile;    Pyrococcus furiosus;   

DOI  :  10.1016/S0014-5793(98)01466-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The E _ms of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the E _m: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution.

【 授权许可】

Unknown