Expression of an oncogenic mutant Gαi2 activates Ras in Rat‐1 fibroblast cells

FEBS Letters

Itoh, Hiroshi¹  Kaziro, Yoshito¹  Edamatsu, Hironori¹ 

[1] Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

关键词: G protein;    gip2;    Ras;    c-Jun N-terminal kinase;    Transformation;    Inducible expression system;    G protein;    heterotrimeric GTP-binding protein;    JNK;    c-Jun N-terminal kinase;    ERK;    extracellular signal-regulated kinase;    IPTG;    isopropyl-1-β-thio-d-galactoside;    GST;    glutathione S-transferase;   

DOI  :  10.1016/S0014-5793(98)01457-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been reported that expression of the active mutant of heterotrimeric GTP-binding protein α subunit Gαi2 transforms Rat-1 cells. However, the Gαi2-mediated mitogenic signaling pathways remain to be elucidated. Here, we demonstrate that inducible expression of the active mutant of Gαi2 (Gαi2(Q205L)) activates Ras and c-Jun N-terminal kinase (JNK) in addition to extracellular signal-regulated kinase (ERK) in Rat-1 cells. Our findings suggest that Ras may play a critical role in the Gαi2-induced transformation and Gαi2 can transduce signals from the Gi-coupled receptor to JNK and ERK in certain types of mammalian cells.

【 授权许可】

Unknown