The involvement of poly(ADP‐ribose) polymerase in the oxidative stress responses in plants

FEBS Letters

Amor, Yehudit²  Inzé, Dirk¹  Levine, Alex²  Babiychuk, Elena¹ 

[1]Laboratory of Genetics, University of Ghent, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium

[2]Department of Plant Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Poly(ADP-ribose) polymerase;    Oxidative stress;    Programmed cell death;    Nicotinamide adenine dinucleotide;    H2O2;   

DOI  :  10.1016/S0014-5793(98)01408-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In plants many biotic and abiotic stresses can cause secondary oxidative stress. Earlier work showed that, depending on the severity of the oxidative stress, plants can activate either cell protective genes or programmed cell death (PCD). Poly(ADP-ribose) polymerase (PARP) has been implicated as one of the enzymes in the apoptotic pathways induced by DNA damaging agents or oxidative stress. We show that in cultured soybean cells, PARP is involved in responses to mild and severe oxidative stresses, by mediating DNA repair and PCD processes, respectively. Addition of PARP inhibitors reduced the degree of cell death triggered by H₂O₂. Two windows of NAD consumption after H₂O₂ treatment were detected. Experiments with transient overexpression of Arabidopsis PARP cDNA promoted DNA repair and inhibited cell death caused by mild oxidative stress. However, following severe stress PARP overexpression increased cell death. Expression of antisense PARP produced the opposite effects: an increase in DNA nicks and inhibition of cell death at high, but not mild doses of H₂O₂.

【 授权许可】

Unknown