Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome 1

FEBS Letters

Shahid, M.¹  Collie, I.T.¹  Dunbar, D.R.¹  Morrow, J.A.¹  Hill, D.R.¹  Walker, G.B.¹ 

[1] Target Discovery Section, Organon Laboratories Limited, Newhouse, Lanarkshire ML1 5SH, UK

关键词: Glycine transporter;    cDNA cloning;    Genomic localization;    Neurotransmitter;    Human spinal cord;    Brain stem;    Cerebellum;    GlyT;    glycine transporter;    NMDA;    N-methyl-d-aspartate;    PCR;    polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    dNTP;    deoxyribonucleotide triphosphate;    DMEM;    Dulbecco's modified Eagle's medium;    FBS;    foetal bovine serum;    HBSS;    Hanks' balanced salts solution;   

DOI  :  10.1016/S0014-5793(98)01390-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We have cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na⁺/Cl⁻-dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose-dependent uptake of glycine with an apparent K _m of 108 μM. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD=8.988) on human chromosome 11p15.1–15.2.

【 授权许可】

Unknown