Inactivation of bacterial respiratory chain enzymes by singlet oxygen

FEBS Letters

Tatsuzawa, Hidetaka⁵  Hori, Kazutoshi²  Fujimori, Ken¹  Kambayashi, Yasuhiro³  Maruyama, Tadashi⁵  Nakano, Minoru³  Sano, Yosiaki³  Misawa, Norihiko⁴ 

[1] Department of Chemistry, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-0006, Japan;Department of Internal Medicine 4, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya 663-8501, Japan;Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories, 351-1 Nishiyokote-cho, Takasaki 370-0021, Japan;Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;Shimizu Laboratories, Marine Biotechnology Institute (MBI), 1900 Sodesi-cho, Shimizu 424-0037, Japan

关键词: Singlet oxygen;    3-(4′-Methyl-1′-naphthyl)propionic acid;    1′;    4′-endoperoxide;    Bactericidal action;    Respiratory chain;    Membrane oxidase;    CLI;    chemiluminescence intensity;    cpm;    count/min;    ETPs;    electron transport particles;    H2O2;    hydrogen peroxide;    HOBr;    hypobromous acid;    HOCl;    hypochlorous acid;    LT;    lycopene transformant;    1O2;    singlet oxygen;    O⋅− 2;    superoxide anion;    MCLA;    2-methyl-6-(p-methoxyphenyl)-3;    7-dihydroimidazo[1;    2-a]pyrazin-3-one;    MPO;    myeloperoxidase;    NEPO;    3-(4′-methyl-1′-naphthyl)propionic acid;    1′;    4′-endoperoxide;    NPA;    3-(4′-methyl-1′-naphthyl)propionic acid;    SOD;    superoxide dismutase;    ATP;    adenosine triphosphate;   

DOI  :  10.1016/S0014-5793(98)01397-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To distinguish the bactericidal action of singlet oxygen (¹O₂) from hypohalous acids, wild-type and lycopene transformant E. coli strains were exposed to each of the oxidants and then bacterial viability was investigated. ¹O₂ was generated by chemical and enzymatic systems at pH 4.5. Exposure of wild-type E. coli to ¹O₂ caused a significant loss of E. coli viability due to inactivation of membrane respiratory chain enzymes by ¹O₂. This action of ¹O₂ could be attenuated by lycopene in the bacterial cell membrane. In the lycopene transformant strain of E. coli, inactivation of NADH oxidase and succinate oxidase by hypohalous acids were significantly suppressed, but E. coli viability was unaffected. Based on these findings, we suggest that phagocytic leukocytes produce ¹O₂ as a major bactericidal oxidant in the phagosome.

【 授权许可】

Unknown