The Na+ and K+ transport deficiency of an E. coli mutant lacking the NhaA and NhaB proteins is apparent and caused by impaired osmoregulation

FEBS Letters

Barquera, Blanca¹  Verkhovsky, Michael I¹  Verkhovskaya, Marina L¹  Wikström, Mårten¹ 

[1] Helsinki Bioenergetics Group and Biocentrum Helsinki, Department of Medical Chemistry, Institute of Biomedical Sciences, P.O. Box 8, University of Helsinki, 00014 Helsinki, Finland

关键词: Sodium/proton antiporter;    Sodium transport;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(98)01380-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cells of the E. coli mutant EP432, which lacks the two Na⁺/H⁺ antiporters, NhaA and NhaB, have been reported to have an impaired sodium transport activity (Harel-Bronstein et al. (1995) J. Biol. Chem. 270, 3816–3822). Here we report that active transport of Na⁺ in EP432 cells can be restored to wild-type levels, either by a high K⁺ concentration or by an increase in the medium osmolarity. We suggest that this mutant is primarily deficient in osmoregulation rather than in cation transport per se.

【 授权许可】

Unknown