Effects of cold acclimation and palmitate on energy coupling in duckling skeletal muscle mitochondria

FEBS Letters

Roussel, Damien¹  Rouanet, Jean-Louis¹  Duchamp, Claude¹  Barré, Hervé¹ 

[1]Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-UCBL, 8, Avenue Rockefeller, F-69373 Lyon Cedex 08, France

关键词: Cold acclimation;    ATP synthesis;    Fatty acid;    Non-shivering thermogenesis;    Muscle mitochondrion;    Δψ;    transmembrane electric potential;    UCP;    uncoupling protein;    BAT;    brown adipose tissue;    CA;    cold-acclimated;    EDTA;    ethylenediamine-N;    N;    N′;    N′-tetraacetic acid;    EGTA;    ethylene glycol-bis(β-aminoethyl ether)-N;    N;    N′;    N′-tetraacetic acid;    FAs;    fatty acids;    FAF-BSA;    fatty-acid-free bovine serum albumin;    NST;    non-shivering thermogenesis;    IFM;    intermyofibrillar mitochondria;    SR;    sarcoplasmic reticulum;    SSM;    subsarcolemmal mitochondria;    TN;    thermoneutral control;   

DOI  :  10.1016/S0014-5793(98)01382-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gastrocnemius subsarcolemmal and intermyofibrillar mitochondria were isolated from 5-week-old cold-acclimated and thermoneutral control ducklings. In vitro respiration (polarography) and ATP synthesis (bioluminescence) were determined at 25°C. Subsarcolemmal mitochondria showed a higher state 4 respiration and lower respiratory control and ADP/O ratio in cold-acclimated than in thermoneutral ducklings. Palmitate decreased the rate of ATP synthesis in both mitochondrial populations to about 30% of maximal but failed to abolish this process even at high concentrations. It is suggested that both expensive ATP synthesis and increased ATP hydrolysis could contribute synergistically to muscle non-shivering thermogenesis in cold-acclimated ducklings.

【 授权许可】

Unknown