Immunohistochemical detection of dityrosine in lipofuscin pigments in the aged human brain

FEBS Letters

Hashizume, Yoshio²  Osawa, Toshihiko⁵  Kato, Yoji¹  Maruyama, Wakako³  Naoi, Makoto⁴ 

[1] School of Humanity for Environment Policy and Technology, Himeji Institute of Technology, Himeji 670-0092, Japan;Institute for Medical Science of Aging, Aichi Medical University, Nagakute-cho, Aichi 480-1195, Japan;Department of Basic Gerontology, National Institute for Longevity Sciences, 36-3 Gengo, Morioka-cho, Obu, Aichi 474-0031, Japan;Department of Brain Sciences, Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Gifu 505-0116, Japan;Department of Applied Biological Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan

关键词: Dityrosine;    Lipofuscin;    Aging;    Oxidation;    MDA;    malondialdehyde;    DOPA;    3;    4-dihydroxyphenylalanine;    KLH;    keyhole limpet hemocyanin;    EDC;    1-ethyl-3-(3-dimethylaminopropyl)carbodiimide;    sulfo-NHS;    N-hydroxysulfosuccinimide;    HPLC;    high-performance liquid chromatography;    FAB-MS;    fast-atom bombardment mass spectrometry;    LC-MS;    liquid chromatography-mass spectrometry;    PBS;    phosphate-buffered saline;    ELISA;    enzyme-linked immunosorbent assay;    HNE;    4-hydroxy-2-nonenal;    GC-MS;    gas chromatography-mass spectrometry;   

DOI  :  10.1016/S0014-5793(98)01372-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lipofuscin is a yellowish brown fluorescent pigment which is sequestered within cytoplasmic granules during aging. To examine the contribution of protein oxidation to lipofuscin accumulation, we performed immunohistochemical detection of dityrosine, which is considered one of the specific markers for protein oxidation, in lipofuscin in the aged human brain using an antibody specific to dityrosine. By characterization using competitive enzyme-linked immunosorbent assay, the specificity of the antibody to dityrosine was confirmed. None of the other tyrosine-related compounds such as l-tyrosine, 3-nitrotyrosine, 3-chlorotyrosine, or 3,4-dihydroxyphenylalanine cross-reacted with the antibody. The anti-dityrosine antibody reacted with lipofuscin granules in the pyramidal neurons of the aged human brain. The results suggest that protein oxidation by free radicals and/or peroxidases may play an important role in lipofuscin accumulation.

【 授权许可】

Unknown