Pyrimidinoceptor potentiation by ATP in NG108‐15 cells

FEBS Letters

Sak, Katrin¹  Uri, Asko¹  Järv, Jaak¹  Kelve, Merike² 

[1] Institute of Chemical Physics, Tartu University, 2 Jakobi Str., EE-2400 Tartu, Estonia;Institute of Chemical Physics and Biophysics, 23 Akadeemia tee, EE-0026 Tallinn, Estonia

关键词: UTP;    UDP;    ATP;    ATP analog;    Pyrimidinoceptor;    Inositol phospholipid hydrolysis;    Extracellular receptor phosphorylation;    ATPγS;    adenosine 5′-O-(3-thiotriphosphate);    βγMeATP;    β;    γ-methyleneadenosine 5′-triphosphate;    BzATP;    3′-O-(4-benzoyl)benzoyl ATP;    dATPαS;    3′-deoxyadenosine 5′-O-(1-thio)triphosphate;    EDTA;    ethylenediamine tetraacetate;    DMEM;    Dulbecco's modified Eagle's medium;    TCA;    trichloroacetic acid;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/S0014-5793(98)01348-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Regulation of inositol phospholipid hydrolysis by UTP and UDP in neuroblastoma×glioma hybrid cell line NG108-15 was potentiated in the presence of ATP. The effect of ATP was dose dependent and shifted the EC₅₀ value for these uracil nucleotides up to three powers of magnitude, having no influence on the maximal value of the response. Adenine nucleotides (ADP, AMP, adenosine 5′-O-(3-thiotriphosphate) (ATPγS), β,γ-methyleneadenosine 5′-triphosphate (βγMeATP), 3′-O-(4-benzoyl)benzoyl ATP (BzATP) and 3′-deoxyadenosine 5′-O-(1-thio)triphosphate (dATPαS)) as well as adenosine, had no influence on the pyrimidinoceptor response. The potentiation effect was abolished by excess of EDTA. The results were in agreement with the hypothesis of pyrimidinoceptor affinity regulation via extracellular phosphorylation of the receptor protein, initiated by ATP. This mechanism may have physiological implication for functioning of uracil nucleotides as endogenous signaling molecules.

【 授权许可】

Unknown