Cardiac myocytes express mRNA for ten RGS proteins: changes in RGS mRNA expression in ventricular myocytes and cultured atria

FEBS Letters

Kardestuncer, Tarik¹  Neer, Eva J¹  Lim, Angela L¹  Wu, Haiguo¹ 

[1] Department of Medicine, Cardiovascular Division, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA

关键词: RGS protein;    G protein;    Heart;    Cardiocyte;    mRNA expression;    RGS;    regulators of G-protein signalling;    ACCITT;    albumin;    carnitine;    creatine;    insulin;    taurine;    triiodothyronine;   

DOI  :  10.1016/S0014-5793(98)01319-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Regulators of G-protein signalling (RGS) are recently identified proteins that shorten the lifetime of the activated G protein. We now show that rat cardiac myocytes express mRNA for at least 10 RGS. The mRNA for RGS-r is barely detectable in rat ventricles, but increases more than 20-fold during the 60- to 90-min process of isolating ventricular myocytes, and after 90 min of culture of atrial pieces in medium with Ca²⁺. Both in myocytes and in atria, the rise in RGS-r is transient. The mRNA for cardiac RGS5, but not RGS-r, is developmentally regulated. These studies suggest that rapid regulation of RGS levels may be a new mechanism that governs how signals are transmitted across the cardiac cell membrane.

【 授权许可】

Unknown