Factors responsible for inhibiting the motility of zoospores of the phytopathogenic fungus Aphanomyces cochlioides isolated from the non‐host plant Portulaca oleracea

FEBS Letters

Hashidoko, Yasuyuki¹  Mizutani, Masanori¹  Tahara, Satoshi¹ 

[1] Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan

关键词: Zoospore motility;    Stimulant;    Repellent;    Motility inhibiting factor;    Portulaca oleracea;    Aphanomyces cochlioides;    EI-MS;    electron impact mass spectrometry;    EtOAc;    ethyl acetate;    FD-MS;    field desorption mass spectrometry;    HR-FAB-MS;    high resolution fast atom bombardment mass spectrometry;    MeOH;    methanol;   

DOI  :  10.1016/S0014-5793(98)01308-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In a survey of plant secondary metabolites regulating the behaviour of Aphanomyces cochlioides zoospores, we found that root extracts of Portulaca oleracea inhibited zoospore motility. Bioassay-directed fractionation of Portulaca constituents revealed that the inhibitory activity was dependent on the interaction of two chemically different factors. These were identified as a phenolic compound, N-trans-feruloyltyramine, which by itself was active as a zoospore stimulant, and an acidic compound, 1-linoleoyl-2-lysophosphatidic acid monomethyl ester, which had zoospore-repellent activity. When Chromosorb W AW particles coated with a mixture of these pure compounds were bioassayed in Petri dishes, the inhibitory effect on zoospore motility was identical with that caused by root tip or root extracts of P. oleracea. Inhibited zoospores rapidly settled to the bottom of the Petri dishes where they initially encysted, and then germinated within 1–2 h. This is the first report of factors which inhibit zoospore motility without killing or bursting the zoospores.

【 授权许可】

Unknown