F420H2:NADP oxidoreductase from Methanobacterium thermoautotrophicum: identification of the encoding gene via functional overexpression in Escherichia coli

FEBS Letters

Berk, Holger¹  Thauer, Rudolf K.¹ 

[1] Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie, Fachbereich Biologie der Philipps-Universität Marburg, Karl-von-Frisch-Straße, D-35043 Marburg, Germany

关键词: Coenzyme F420;    NADP-binding motif;    F420-dependent NADP reductase;    Methanogenic archaeon;    Methanobacterium thermoautotrophicum;   

DOI  :  10.1016/S0014-5793(98)01288-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

F₄₂₀H₂:NADP oxidoreductase is found in methanogenic, sulfate-reducing and halophilic archaea and also in some bacteria. The putative gene encoding the enzyme was cloned from Methanobacterium thermoautotrophicum (strain Marburg) and heterologously expressed in Escherichia coli. The overproduced active enzyme was purified, characterized and crystallized.

【 授权许可】

Unknown