Inhibition of Ca2+‐independent phospholipase A2 by bromoenol lactone attenuates prostaglandin generation induced by interleukin‐1β and dibutyryl cAMP in rat mesangial cells

FEBS Letters

Akiba, Satoshi¹  Hayama, Misako¹  Sato, Takashi¹ 

[1] Department of Pathological Biochemistry, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan

关键词: Phospholipase A2;    Prostaglandin;    Bromoenol lactone;    Rat mesangial cell;    BEL;    bromoenol lactone;    PLA2;    phospholipase A2;    cPLA2;    cytosolic PLA2;    iPLA2;    Ca2+-independent PLA2;    sPLA2;    secretory PLA2;    PGE2;    prostaglandin E2;   

DOI  :  10.1016/S0014-5793(98)01236-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytokine-induced prostaglandin generation in rat mesangial cells has been suggested to be dependent on the expression of secretory phospholipase A₂ (sPLA₂). In the present study, we investigated the possible involvement of Ca²⁺-independent phospholipase A₂ (iPLA₂) in the generation. The results showed that bromoenol lactone, a relatively selective iPLA₂ inhibitor, significantly attenuated prostaglandin E₂ generation induced by interleukin-1β and dibutyryl cAMP in parallel with the inhibition of iPLA₂ activity. However, the inhibitor did not affect sPLA₂ release upon stimulation, activities of sPLA₂ or cytosolic phospholipase A₂, or Ca²⁺ ionophore-induced arachidonic acid liberation. These results suggest that prostaglandin E₂ generation upon stimulation may be partially mediated by iPLA₂ in addition to sPLA₂.

【 授权许可】

Unknown