Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two‐hybrid analysis and functional complementation tests of yeast mutants

FEBS Letters

Giraudat, Jérôme³  Ichimura, Kazuya⁴  Mizoguchi, Tsuyoshi⁴  Morris, Peter¹  Matsumoto, Kunihiro²  Irie, Kenji²  Shinozaki, Kazuo⁴ 

[1] Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, UK;Department of Molecular Biology, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan;Institut des Sciences Végétales, Centre National de la Recherche Scientifique UPR 40, Avenue de la Terrasse, 91198, Gif sur Yvette Cédex, France;Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan

关键词: Mitogen-activated protein kinase cascade;    Yeast two-hybrid;    trans-Complementation;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(98)01197-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A possible MAP kinase (MAPK) cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK) and MEK1 (a MAPKK) and interactions between MEK1 and ATMEKK1 (a MAPKKK) were detected by using the 2-hybrid system. A growth defect of the yeast mpk1Δ mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2Δ mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants.

【 授权许可】

Unknown