Identification of a Torpedo homolog of Sam68 that interacts with the synapse organizing protein rapsyn

FEBS Letters

Worley, Paul¹  Lanahan, Anthony¹  Huganir, Richard L.¹  Fung, Eric T.¹ 

[1] Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, 904A PCTB, 725 North Wolfe Street, Baltimore, MD 21205, USA

关键词: Neuromuscular junction;    Rapsyn;    Sam68;    nAChR;    nicotinic acetylcholine receptor;    DABCO;    1;    4-diazabicyclo[2;    2;    2]-octane;    SAM68;    src-associated in mitosis;    ARIA;    acetylcholine receptor-inducing activity;    TBS;    Tris-buffered saline;    PBS;    phosphate-buffered saline;    PMSF;    phenylmethylsulfonyl fluoride;    GST;    glutathione S-transferase;    PAGE;    polyacrylamide gel electrophoresis;    PKA;    protein kinase A;    PKC;    protein kinase C;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(98)01151-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10 000/μm². The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction.

【 授权许可】

Unknown