FEBS Letters | |
Characterization of genes for two‐component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana | |
Urao, Takeshi1  Miyata, Shin-ichi1  Yamaguchi-Shinozaki, Kazuko1  Shinozaki, Kazuo2  | |
[1] Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan;Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan | |
关键词: Two-component system; HPt domain; tissue-specific expression; Arabidopsis thaliana; | |
DOI : 10.1016/S0014-5793(98)01188-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Three cDNAs that encode two-component phosphorelay-mediator-like proteins were cloned from Arabidopsis thaliana. Putative proteins (ATHP1–3) contain an HPt (istidine-containing hospho ransfer)-like domain with a conserved histidine and some invariant residues that are involved in phosphorelay. Growth retardation of YPD1-disrupted yeast cells was reversed with ATHPs, which indicates that ATHPs function as phosphorelay mediators in yeast cells. The ATHP genes are expressed more in roots than in other tissues, similar to the expression of genes for a sensor histidine kinase, ATHK1, and response regulators ATRR1–4. These results suggest that ATHPs function as two-component phosphorelay mediators between sensor histidine kinase and response regulators in Arabidopsis.
【 授权许可】
Unknown
【 预 览 】
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