Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron‐responsive element

FEBS Letters

Söderhäll, Kenneth³  Melefors, Öjar¹  Ekengren, Sophia²  Lind, Maria I.³ 

[1]Microbiology and Tumor Biology Center (MTC), Karolinska Institute, 171 77 Stockholm, Sweden

[2]Department of Developmental Biology, Stockholm University, 106 91 Stockholm, Sweden

[3]Department of Physiological Mycology, Uppsala University, Villavägen 6, 752 36 Uppsala, Sweden

关键词: Alternative RNA splicing;    Ferritin;    Iron;    Iron-responsive element;    Drosophila melanogaster;   

DOI  :  10.1016/S0014-5793(98)01186-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several mRNAs encoding the same ferritin subunit of Drosophila melanogaster were identified. Alternative RNA splicing and utilisation of different polyadenylation sites were found to generate the transcripts. The alternative RNA splicing results in ferritin transcripts with four unique 5′ untranslated regions. Only one of them contains an iron-responsive element. The iron-responsive element was found to bind in vitro specifically to human recombinant iron regulatory protein 1. Furthermore, the ferritin subunit mRNAs are differentially expressed during development. Our data provides the first molecular evidence that the presence of iron-responsive element in a ferritin mRNA is regulated by alternative RNA splicing.

【 授权许可】

Unknown