Ancient gene duplication and domain shuffling in the animal cyclic nucleotide phosphodiesterase family

FEBS Letters

Kuma, Kei-ichi¹  Suga, Hiroshi¹  Koyanagi, Mitsumasa¹  Miyata, Takashi¹  Iwabe, Naoyuki¹  Hoshiyama, Daisuke¹  Ono, Kanako¹ 

[1] Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan

关键词: Phosphodiesterase;    Sponge;    Gene duplication;    Domain shuffling;    Phylogenetic tree;    Gene diversity;    Evolution;    PDE;    phosphodiesterase;    Gα;    G protein α subunit;    PTK;    protein tyrosine kinase;    Myr;    million year;   

DOI  :  10.1016/S0014-5793(98)01150-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The animal cyclic nucleotide phosphodiesterases (PDEs) comprise at least seven subtypes, PDE1–7, which differ from each other in domain organization and primary function, and they diverged from an ancestral gene by gene duplication and domain shuffling during animal evolution. To obtain rough estimates for the divergence times of these subtypes, cloning of PDE cDNAs from Ephydatia fluviatilis (freshwater sponge) by RT-PCR was carried out. We obtained four cDNAs, EFPDE1, EFPDE2, EFPDE3, and EFPDE4, which are possibly homologs of the vertebrate PDE1, PDE2, PDE3, and PDE4, respectively, judging from the sequence similarity, domain organization, and branching pattern in the phylogenetic tree. The phylogenetic tree of the PDE family revealed that most gene duplications and domain shufflings that gave rise to different subtypes had been completed in the early evolution of animals before the separation of sponges and eumetazoans.

【 授权许可】

Unknown