Telomerase from Saccharomyces cerevisiae contains several protein subunits and may have different activities depending on the protein content

FEBS Letters

Favre, Alain²  Petrov, Andrey V³  Dokudovskaya, Svetlana S³  Bogdanov, Alexey A³  Lavrik, Olga I¹  Dontsova, Olga A³  Sokolov, Kirill A³ 

[1] Novosibirsk Institute of Bioorganic Chemistry RAS, Novosibirsk, Russia;Institut Jacques Monod, Paris, France;Department of Chemistry, Moscow State University, Moscow 119899, Russia

关键词: Telomerase;    Yeast;    Processivity;    Nuclease activity;   

DOI  :  10.1016/S0014-5793(98)01091-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Telomerase is a ribonucleoprotein responsible for maintaining telomeres during the cell cycle [1, 2]. Here we describe a two-step purification procedure for the Saccharomyces cerevisiae telomerase complex. We have found that the properties (processivity, nuclease activity) of telomerase depend on the isolation procedure. Using a cross-linking approach, we have revealed several proteins that could be components of the telomerase complex. Furthermore, spectra of cross-linked proteins differ in processive and non-processive telomerase complexes.

【 授权许可】

Unknown