The vascular endothelial growth factor mRNA contains an internal ribosome entry site

FEBS Letters

Goodall, Gregory J²  Dibbens, Justin A²  Vadas, Mathew A²  Damert, Annette¹  Miller, Diane L²  Risau, Werner¹ 

[1] Max Planck Institut für Physiologische und Klinische Forshung, W.G. Kerckhoff Institut, Abteilung Molekulare Zellbiologie, Parkstraße 1, 61231 Bad Neuheim, Germany;Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide, S.A. 5000, Australia

关键词: Vascular endothelial growth factor;    Translation;    5′-Untranslated region;    Ribosome;    IRES;    internal ribosome entry site;    UTR;    untranslated region;    VEGF;    vascular endothelial growth factor;   

DOI  :  10.1016/S0014-5793(98)01025-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vascular endothelial growth factor (VEGF), an essential regulator of angiogenesis during early development as well as during the growth of solid tumours, bears an unusually large 5′ untranslated region (5′-UTR) in the mRNA of over 1000 nucleotides. We found that the VEGF 5′-UTR, despite being GC-rich and containing an upstream short open reading frame, promotes efficient translation of a luciferase reporter. The VEGF 5′-UTR also allowed translation of luciferase from a dicistronic mRNA when placed between the two cistrons, demonstrating that it contains an internal ribosome entry site. Deletion analysis indicated that the IRES resides towards the 3′ end of the 5′-UTR.

【 授权许可】

Unknown