Peroxisome proliferator‐activated receptor γ1 (PPAR‐γ1) as a major PPAR in a tissue in which estrogen induces peroxisome proliferation

FEBS Letters

Kolattukudy, Pappachan E.¹  Ma, Hongwen¹  Tam, Quach Thanh¹ 

[1] The Ohio State University, Neurobiotechnology Center and Departments of Biochemistry and Medical Biochemistry, 206 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA

关键词: Peroxisome proliferator-activated receptor γ;    Uropygial gland;    cDNA cloning;    Peroxisome proliferator;    Transfection;   

DOI  :  10.1016/S0014-5793(98)01017-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Estradiol administration induces peroxisome proliferation and the production of 3-hydroxy fatty acid pheromones in the uropygial glands of the duck, but not in the goose gland, which does not produce such pheromones. We isolated a peroxisome proliferator-activated receptor (PPAR)γ1 cDNA from a duck uropygial gland cDNA library. Northern blots revealed two transcripts, PPARγ1 and γ2, and showed that PPARγ was expressed at higher levels than PPARα in the uropygial gland of the duck. Although PPARγ2 was expressed in both duck and goose uropygial gland, PPARγ1 was expressed only in the duck gland, which responds to estrogen by peroxisome proliferation. In NIH 3T3 transfected cells, PPARγ1 was activated by peroxisome proliferators such as Wy-14 643, clofibric acid and Ly-171 883 causing induction of the target marker gene. By cotransfection with a plasmid containing α-cis-retinoic acid receptor RXRα, the induction increased up to 9-fold. These results suggest that PPARγ1 may be involved in peroxisome proliferation while PPARγ2 may be involved in lipid metabolism.

【 授权许可】

Unknown