The extended packaging sequence of MoMLV contains a constitutive mRNA nuclear export function

FEBS Letters

King, Jason A.¹  Schulz, Thomas F.²  Schirrmacher, Volker¹  Bridger, Joanna M.³  Lichter, Peter³  Khazaie, Khashayarsha¹  Gounari, Fotini¹ 

[1] Department of Cellular Immunology, German Cancer Research Centre, Im Neuenheimer Feld 280, Heidelberg 69120, Germany;Department of Medical Microbiology and Genito Urinary Medicine, Royal Liverpool University Hospital, Duncan Building, Liverpool L69 3BX, UK;Department of Organisation of the Complex Genome, German Cancer Research Centre, Im Neuenheimer Feld 280, Heidelberg 69120, Germany

关键词: mRNA nuclear export;    Moloney murine leukemia virus;    Extended packaging sequence;    Human T-cell leukemia virus-1;    Rex;   

DOI  :  10.1016/S0014-5793(98)00948-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present report shows that incorporation of defined sequences from the Moloney murine leukaemia virus (MoMLV) into Rex dependent expression vectors based on the human T-cell leukaemia virus (HTLV-1) allows Rex independent gene expression. Deletion mutagenesis of the MoMLV derived sequences allowed this function to be localised to a 312 nt length sequence overlapping the MoMLV gag p15/p12 open reading frame. This ‘extended packaging sequence’ has been reported to markedly increase the titre of in vitro packaged retroviral vectors. Using fluorescent in situ hybridisation combined with confocal microscopy we show that the 312 nt element can replace Rex mediated nuclear export and expression of transcripts containing HTLV-1 cis acting repressive elements. Our observations are consistent with the extended packaging sequence of MoMLV exerting a constitutive mRNA nuclear export function.

【 授权许可】

Unknown