Observing the deoxy myoglobin and hemogobin signals from rat myocardium in situ

FEBS Letters

Kreutzer, Ulrike¹  Tran, Tuan-Khanh¹  Jue, Thomas¹ 

[1] Department of Biological Chemistry, University of California Davis, Davis, CA 95616-8635, USA

关键词: Myoglobin;    Hemoglobin;    Nuclear magnetic resonance;    Myocardium;    DSS;    dimethylsilapentane sulfonate;    Mb;    myoglobin;    Hb;    hemoglobin;    pO2;    partial pressure of oxygen;   

DOI  :  10.1016/S0014-5793(98)01001-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹H NMR proximal histidyl N_δH signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO₂ is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O₂, the erythrocyte Hb is partially desaturated and yields the α and β proximal histidyl N_δH signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO₂.

【 授权许可】

Unknown