Promoter‐independent regulation of cell‐specific dopamine receptor expression

FEBS Letters

Wong, Albert H.C¹  Schoots, Oscar¹  Knapp, Mark¹  Van Tol, Hubert H.M¹  Guan, H.C¹ 

[1] Laboratory of Molecular Neurobiology, Clarke Institute of Psychiatry, University of Toronto, Toronto, Ont. M5T 1R8, Canada

关键词: Dopamine;    Adenovirus;    Expression;    D2 dopamine receptor;    D4 dopamine receptor;    Cell line;    Ligand binding;    Cyclic AMP;   

DOI  :  10.1016/S0014-5793(98)00961-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we describe the construction of recombinant adenoviruses expressing dopamine D2 and D4 receptors, and their ability to mediate high levels of heterologous expression in a variety of cell types in vitro and in vivo for at least 7 days post infection. These experiments demonstrated that maximum receptor expression is achieved generally within 24 h and remains constant thereafter. Maximum expression levels were highly variable between cell lines and dependent on infection efficiency and promoter strength. Correction for these two variables revealed differences in relative expression levels between cell lines varying by two orders of magnitude. Our results indicate that in addition to gene transcription, post-transcriptional mechanisms play a dominant role in determining dopamine receptor levels in this system.

【 授权许可】

Unknown