Modification of pigment composition in the isolated reaction center of photosystem II

FEBS Letters

Scheer, Hugo¹  Zehetner, Andrea¹  Gall, Bernhard¹  Scherz, Avigdor² 

[1] Botanisches Institut der Universität München, Menzingerstr. 67, D-80638 Munich, Germany;Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, Israel

关键词: Photosynthesis;    Photosystem II;    Reaction center;    D1-D2-cytochrome b-559 complex;    Pigment modification;    Acetyl-Chl;    [3-acetyl]-chlorophyll a;    BA;    B;    binding pockets of the monomeric BChls in the bacterial RC;    BChl;    bacteriochlorophyll a;    BPhe;    bacteriopheophytin a;    CHAPS;    3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate;    Chl;    chlorophyll a;    Chl′;    132 epimer of Chl;    DM;    dodecyl-β-d-maltoside;    HA;    B;    binding pockets of the monomeric BPhes in the bacterial RC;    PSII-RC;    photosystem II reaction center=D1-D2-cytochrome b 559 complex;    RC;    reaction center;    Zn-Chl;    [Zn]-chlorophyll a;   

DOI  :  10.1016/S0014-5793(98)00956-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pigment content of isolated reaction centers of photosystem II was modified using an exchange protocol similar to that used for purple bacterial reaction centers. With this method, which is based on incubation of reaction centers at elevated temperature with an excess of chemically modified pigments, it was possible to incorporate [3-acetyl]-chlorophyll a and [Zn]-chlorophyll a into photosystem II reaction centers. Pigment exchange has been verified by absorption, circular dichroism and fluorescence spectroscopy, and quantitated by HPLC analysis of pigment extracts.

【 授权许可】

Unknown