Ligand‐induced endocytosis of the asialoglycoprotein receptor: evidence for heterogeneity in subunit oligomerization

FEBS Letters

Spiess, Martin¹  Bider, Marc D¹ 

[1] Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

关键词: Asialoglycoprotein receptor;    Endocytosis;    Hepatic lectin;    Receptor downregulation;    ASGP;    asialoglycoprotein;    ASOR;    asialoorosomucoid;    CRD;    carbohydrate recognition domain;    LDL;    low density lipoprotein;    MGP;    monoglycosylated peptide;    SSHPP;    sulfosuccinimidyl-3-(4-hydroxyphenyl) propionate;   

DOI  :  10.1016/S0014-5793(98)00947-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hepatic asialoglycoprotein receptor, a non-covalent hetero-oligomer of two subunits, is a constitutively cycling endocytic receptor. However, the ligand asialoorosomucoid caused downregulation of up to 40% of surface binding sites and a twofold increase in internalization rate. This was not the result of receptor crosslinking, since monovalent ligands had the same effect. Ligand binding thus appears to transmit a signal to the cytosolic portion of the receptor not unlike in signaling receptors. The two subunits were endocytosed at different average rates lower than that of ligand, indicating heterogeneity in oligomer formation and potentially in ligand specificity.

【 授权许可】

Unknown