Expression of pICln in Escherichia coli gives a strong tolerance to hypotonic stress

FEBS Letters

Kobayashi, Akira¹  Tashima, Yohtalou¹  Itoh, Hideaki¹  Tao, Guo-Zhong¹ 

[1] Department-2 of Biochemistry, School of Medicine, Akita University, Hondo 1-1-1, Akita 010-8543, Japan

关键词: Putative volume (or osmo)-sensitive Cl− channel;    Channel regulator;    pICln protein;    Expression;    Hypotonic tolerance;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(98)00946-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We amplified the coding region DNA sequence from a rat renal pI_Cln cDNA by PCR and expressed the protein in Escherichia coli cells. The cells were exposed to hypotonic conditions followed by spreading them onto LB plates for subsequent colony survival assay. The present study demonstrated that the cells expressing pI_Cln exhibit a strong resistance to hypotonic stress. Moreover, the resistance was specifically inhibited by extracellular ATP and some anion channel inhibitors. These findings indicate that the expression of pI_Cln directly confers tolerance to hypotonic stress, and pI_Cln is concluded to be an important molecule for cell-volume regulation.

【 授权许可】

Unknown