Fe65 and the protein network centered around the cytosolic domain of the Alzheimer's β‐amyloid precursor protein

FEBS Letters

Zambrano, Nicola¹  Minopoli, Giuseppina¹  De Candia, Paola¹  De Renzis, Stefano¹  Faraonio, Raffaella¹  Russo, Tommaso¹ 

[1] Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, via S. Pansini 5, 80131 Naples, Italy

关键词: Fe65;    β-Amyloid precursor protein;    Protein-protein interaction;    Alzheimer;    Amyloid;   

DOI  :  10.1016/S0014-5793(98)00941-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A distinctive tract of all the forms of Alzheimer's disease is the extracellular deposition of a 40–42/43 amino acid-long peptide derived from the so-called β-amyloid precursor protein (APP). This is a membrane protein of unknown function, whose short cytosolic domain has been recently demonstrated to interact with several proteins. One of these proteins, named Fe65, has the characteristics of an adaptor protein; in fact, it possesses three protein-protein interaction domains: a WW domain and two PID/PTB domains. The interaction with APP requires the most C-terminal PID/PTB domain, whereas the WW domain is responsible for the interaction with various proteins, one of which was demonstrated to be the mammalian homolog of the Drosophila enabled protein (Mena), which in turn interacts with the cytoskeleton. The second PID/PTB domain of Fe65 binds to the CP2/LSF/LBP1 protein, which is an already known transcription factor. The other proteins interacting with the cytosolic domain of APP are the G_o heterotrimeric protein, APP-BP1 and X11. The latter interacts with APP through a PID/PTB domain and possesses two other protein-protein interaction domains. The small size of the APP cytodomain and the overlapping of its regions involved in the binding of Fe65 and X11 suggest the existence of competitive mechanisms regulating the binding of the various ligands to this cytosolic domain. In this short review the possible functional roles of this complex protein network and its involvement in the generation of Alzheimer's phenotype are discussed.

【 授权许可】

Unknown