Evidence for a leukotriene A4 hydrolase in Xenopus laevis skin exudate

FEBS Letters

Cohen, Paul¹  Barre, Nicole¹  Clamagirand, Christine¹  Cadel, Sandrine¹ 

[1] Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, Unité Mixte de Recherche 7631 du CNRS, Université Pierre et Marie Curie, 96 Boulevard Raspail, F-75006 Paris, France

关键词: Aminopeptidase;    Leukotriene A4 hydrolase;    Leukotriene B4;    Dactylysin;    Eicosanoid;    (Xenopus laevis);   

DOI  :  10.1016/S0014-5793(98)00885-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Leukotriene A₄ hydrolase is a cytosolic metalloenzyme of the arachidonic acid biosynthetic pathway responsible for leukotriene A₄ conversion into leukotriene B₄. In addition to its epoxide hydrolase properties, this enzyme exhibits an aminopeptidase activity which was used as an assay to monitor the purification of a novel form of leukotriene A₄ hydrolase from Xenopus laevis skin exudate. This 70 kDa, secreted, form of leukotriene A₄ hydrolase was identified by immunochemical cross-reactivity with anti-human leukotriene A₄ hydrolase antibodies and by its capacity to convert leukotriene A₄ into leukotriene B₄. Moreover this enzyme produced a second metabolite which could be the leukotriene B₄ isomer 5S,12R-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid, previously shown by Strömberg et al. (Eur. J. Biochem. 238 (1996) 599–605) to be formed by incubation of the leukotriene A₄ with amphibian tissue extracts. Partial amino acid sequencing of peptides generated by endolysin C fragmentation of the purified enzyme confirmed the presence, in X. laevis skin secretions, of a related but distinct form of leukotriene A₄ hydrolase which is likely to be responsible for the production of these eicosanoid metabolites of leukotriene A₄.

【 授权许可】

Unknown