XAS characterization of the active sites of novel intradiol ring‐cleaving dioxygenases: hydroxyquinol and chlorocatechol dioxygenases

FEBS Letters

Golovleva, Ludmila A²  Solyanikova, Inna P²  Mangani, Stefano¹  Briganti, Fabrizio³  Scozzafava, Andrea³  Pedocchi, Luca³  Jadan, Andrea P² 

[1] Dipartimento di Chimica, Università di Siena, Pian dei Mantellini 44, I-53100 Siena, Italy;Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia;Laboratorio di Chimica Inorganica e Bioinorganica, Dipartimento di Chimica, Università degli Studi di Firenze, Via Gino Capponi 7, I-50121 Florence, Italy

关键词: Chlorocatechol 1;    2-dioxygenase;    Extended X-ray absorption fine structure;    Hydroxyquinol 1;    2-dioxygenase;    Intradiol ring-cleaving dioxygenase;    Nocardiodes simplex;    Rhodococcus erythropolis;    ClC1;    2O;    chlorocatechol 1;    2-dioxygenase;    EXAFS;    extended X-ray absorption fine structure;    HQ1;    2O;    hydroxyquinol 1;    2-dioxygenase;    Hydroxyquinol;    1;    2;    4-trihydroxybenzene;    XAS;    X-ray absorption spectroscopy;   

DOI  :  10.1016/S0014-5793(98)00884-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intradiol cleaving dioxygenases hydroxyquinol 1,2-dioxygenase (HQ1,2O) from Nocardiodes simplex 3E, chlorocatechol 1,2-dioxygenase (ClC1,2O) from Rhodococcus erythropolis 1CP, and their anaerobic substrate adducts (hydroxyquinol-HQ1,2O and 4-chlorocatechol-ClC1,2O) have been characterized through X-ray absorption spectroscopy. In both enzymes the iron(III) is pentacoordinated and the distance distribution inside the Fe(III) first coordination shell is close to that already found in the extensively characterized protocatechuate 3,4-dioxygenase. The coordination number and the bond lengths are not significantly affected by the substrate binding. Therefore it is confirmed that the displacement of a protein donor upon substrate binding has to be considered a general step valid for all intradiol dioxygenases.

【 授权许可】

Unknown