Towards a mutant analysis of the tertiary structures of functional DNA‐binding motifs

FEBS Letters

Müller-Hill, Benno¹  Barker, Andrew¹ 

[1] Institut für Genetik der Universität zu Köln, Weyertal 121, D-50931 Köln, Germany

关键词: Transcription factor;    Recognition helix;    Induced fit;    Unstructured protein region;    Cold sensitive mutant;    Heat sensitive mutant;   

DOI  :  10.1016/S0014-5793(98)00797-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcription factors have specific regions, often α helices, with which they recognise DNA. These regions are more or less disordered off DNA. Some examples are listed here. However, a detailed mutant analysis of this phenomenon is missing. It could show to what extent DNA binding in vitro and in vivo is harmed when such a region is artificially made rigid by suitable substitutions and could reveal how much transcription factors have improved by having been selected to carry unstable recognition domains.

【 授权许可】

Unknown