Glucose stimulates the activation domain potential of the PDX‐1 homeodomain transcription factor

FEBS Letters

Serup, Palle²  Peshavaria, Mina¹  Stein, Roland¹  Pedersen, Anette A²  Madsen, Ole D²  Philippe, Jacques³  Petersen, Helle V² 

[1] Department of Molecular Physiology and Biopysics, Vanderbilt Medical Center, Nashville, TN 37232, USA;Hagedorn Research Laboratory, Niels Steensens Vej 6, Gentofte 2820, Denmark;Clinical Diabetology, Department of Medicine, CMU, 1, rue Michel Servet, CH-1211 Geneva 4, Switzerland

关键词: PDX-1;    Glucose;    Transcription;    Insulin;   

DOI  :  10.1016/S0014-5793(98)00776-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucose-stimulated expression of the insulin gene in β cells is mediated by the PDX-1 transcription factor. In this report, we show that stimulation results from effects on activation and DNA-binding potential. Thus, glucose specifically stimulated expression in MIN6 β cells from chimeras of PDX-1 and the GAL4 DNA-binding domain which spanned the N-terminal PDX-1 activation domain located between amino acids 1 to 79. GAL4:PDX activity was induced over physiological glucose concentrations and was also regulated by effectors of this response. The level of endogenous PDX-1 binding and phosphorylation were also induced under these conditions. We discuss how changes in PDX-1 phosphorylation may influence activity in glucose-treated β cells.

【 授权许可】

Unknown