Effects of peroxynitrite on plasma components of the reverse cholesterol transport pathway

FEBS Letters

Vinogradov, Dimitri V¹  Graham, Annette¹  Owen, James S¹ 

[1] Department of Biochemistry and Molecular Biology, and Department of Medicine, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, UK

关键词: Atherogenesis;    Macrophage;    High-density lipoprotein;    Cholesterol efflux;    Peroxynitrite;    apoAI;    apolipoprotein AI;    apoAII;    apolipoprotein AII;    CETP;    cholesteryl ester transfer protein;    HDL;    high-density lipoprotein;    LCAT;    lecithin:cholesterol acyltransferase;    Lp;    lipoprotein;    LDL;    low-density lipoprotein;    ONOO⋅;    peroxynitrite;    RCT;    reverse cholesterol transport;   

DOI  :  10.1016/S0014-5793(98)00785-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Elimination of cholesterol from arterial tissue, crucial in limiting atherogenesis, may be achieved via high-density lipoprotein (HDL)-mediated reverse cholesterol transport (RCT); components of this pathway can be modulated by oxidative stress. Here we have examined the relations between cholesterol efflux, esterification and transfer in human plasma treated with the powerfully reactive nitrogen species, peroxynitrite. Cellular cholesterol efflux to whole plasma, or to peroxynitrite-modified HDL₃, was relatively insensitive to peroxynitrite, as was the transfer of esterified cholesterol. However, plasma cholesterol esterification, via lecithin:cholesterol acyltransferase (LCAT), was markedly inhibited, both directly and indirectly, by peroxynitrite treatment, implying inefficient RCT follows HDL sequestration of cellular cholesterol.

【 授权许可】

Unknown