Inferences about the catalytic domain of P‐type ATPases from the tertiary structures of enzymes that catalyze the same elementary reaction

FEBS Letters

Kasho, Vladimir N¹  Faller, Larry D¹  Smirnova, Irina N¹ 

[1] CURE: Digestive Diseases Research Center, Department of Medicine, University of California at Los Angeles School of Medicine, and Wadsworth Division, Department of Veterans Affairs Medical Center, Building 115, Room 324, 11301 Wilshire Boulevard, West Los Angeles, CA 90073, USA

关键词: Active transport;    Enzyme structure;    Phosphate transfer;    P-type pump;    AAA family;    ABC transporter;   

DOI  :  10.1016/S0014-5793(98)00760-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The machinery to catalyze elementary reactions is conserved, and the number of solved enzyme structures is increasing exponentially. Therefore, structures of enzymes that catalyze phosphate transfer are reviewed, and a supersecondary structure connecting the Walker A sequence to another sequence containing functional amino acids is proposed as an additional signature for the active site. The new signature is used to infer the identity of the P-loop in P-type biological pumps and may be useful in predicting targets for site-directed mutagenesis in other enzymes of unknown structure like the AAA family and ABC transporters.

【 授权许可】

Unknown