Interferon regulatory factor‐1 is a major regulator of epidermal growth factor receptor gene expression

FEBS Letters

Proctor, Kimberle N¹  Bergel, Michael²  Rubinstein, Yaffa R¹  Johnson, Alfred C¹  Murphy, Barbara¹ 

[1] Laboratory of Molecular Biology, Division of Basic Sciences, National Cancer Institute, NIH, Building 37 Room 2D18, 37 Convent Drive MSC-4255, Bethesda, MD 20892, USA;Laboratory of Molecular Carcinogenesis, Division of Basic Sciences, National Cancer Institute, Bethesda, MD 20892, USA

关键词: Epidermal growth factor receptor;    Promoter;    Gene regulation;    Interferon;    DNA binding;   

DOI  :  10.1016/S0014-5793(98)00774-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Overexpression of the epidermal growth factor receptor (EGFR) occurs in many tumors and in breast cancer correlates with poor prognosis for treatment. Here, we report that interferon regulatory factor-1 (IRF-1) induces EGFR promoter activity up to 200-fold compared to 3–10-fold induction by other regulators. The region of the promoter that is required for this induction was defined using deletion mutants. In addition, we found that IRF-1 and tricostatin A, a deacetylase inhibitor, have a synergistic effect on EGFR promoter activity. This indicates that the increase in EGFR promoter activity by IRF-1 may also involve changes in chromatin structure. These results identify IRF-1 as a major regulator of EGFR gene expression.

【 授权许可】

Unknown