Differential actin organization by vinculin isoforms: implications for cell type‐specific microfilament anchorage

FEBS Letters

Weiss, Elisabeth E¹  Rüdiger, Manfred¹  Schwienbacher, Christine¹  Jockusch, Brigitte M¹  Korneeva, Nadja¹ 

[1] Cell Biology, Zoological Institute, Technical University Braunschweig, D-38092 Braunschweig, Germany

关键词: Vinculin;    Metavinculin;    Actin organization;    Myofibril anchorage;   

DOI  :  10.1016/S0014-5793(98)00723-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vinculin is found in all adherens junctions, while metavinculin, a larger splice variant, is coexpressed with vinculin only in smooth and cardiac muscle. To understand the significance of metavinculin expression, we compared ligand binding between turkey vinculin and metavinculin. Residues 1–258 were found essential for head-tail interactions in both proteins. The tail domains (VT and MVT, respectively) both bind to F-actin. However, while VT bundles F-actin, MVT generates highly viscous F-actin webs. In transfected PtK2 cells, VT causes F-actin needles or coils, while MVT-expressing cells display a diffuse F-actin distribution. Thus, the MVT-specific insert induces an F-actin supraorganization different from the VT-based form, suggesting that metavinculin has a specific role in muscle.

【 授权许可】

Unknown