Mosaic Qβ coats as a new presentation model

FEBS Letters

Pumpens, Paul¹  Strelnikova, Anna¹  Ose, Velta¹  Cielens, Indulis¹  Vasiljeva, Inta¹  Kazaks, Andris¹  Kozlovska, Tatjana¹ 

[1] Biomedical Research and Study Centre, University of Latvia, Ratsupites Str. 1, Riga, Latvia

关键词: Phage Qβ;    Coat protein UGA suppression;    A1 extension;    Capsid assembly;    Hepatitis B virus preS1;    Immunogenicity;   

DOI  :  10.1016/S0014-5793(98)00716-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The new protein carrier was developed on the basis of recombinant RNA phage Qβ capsid. C-terminal UGA extension of the short form of Qβ coat, so-called A1 extension, served as a target for presentation of foreign peptides on the outer surface of mosaic Qβ particles. In conditions of enhanced UGA suppression, the proportion of A1-extended to short coats in mosaic particles dropped from 48% to 14%, with an increase of the length of A1 extension. A model insertion, short preS1 epitope 31-DPAFR-35 of hepatitis B surface antigen, demonstrated superficial location on the mosaic Qβ particles and ensured specific antigenicity and immunogenicity.

【 授权许可】

Unknown