Guanine nucleotides protect against kainate toxicity in an ex vivo chick retinal preparation

FEBS Letters

Barat, Ana¹  Ramı́rez, Galo¹  Burgos, Javier S¹  Souza, Diogo O² 

[1]Centro de Biologı́a Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, 28049 Madrid, Spain

[2]Departamento de Bioquı́mica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90035-003, Brazil

关键词: Kainate;    Guanosine monophosphate;    Guanine nucleotide;    Excitotoxicity;    Neuroprotection;    Chick retina;    ADPβS;    adenosine 5′-O-(2-thiodiphosphate);    AMPA;    α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate;    AP7;    2-amino-7-phosphonoheptanoate;    AppHNp;    5′-adenylyl-imidodiphosphate;    DNQX;    6;    7-dinitroquinoxaline-2;    3-dione;    EAA;    excitatory amino acids;    GDPβS;    guanosine 5′-O-(2-thiodiphosphate);    GN;    guanine nucleotide;    GppNHp;    5′-guanylyl-imidodiphosphate;    KA;    kainic acid;    KBSS;    Krebs balanced salt solution;    LDH;    lactate dehydrogenase;    MK-801;    (+)-5-methyl-10;    11-dihydro-5H-dibenzo-[a;    d]cyclohepten-5;    10-imine maleate;    NMDA;    N-methyl-d-aspartate;   

DOI  :  10.1016/S0014-5793(98)00651-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ex vivo preparations of chick neural retina have been successfully used in the assessment of excitotoxicity and in the evaluation of the protective effects of glutamate antagonists. Using a variation of this approach, and measuring the acute and delayed toxic effects of kainate (KA) in terms of lactate dehydrogenase release, we have shown that guanine nucleotides behave as effective neuroprotecting agents. The anti-excitotoxic potency of guanine nucleotides (in the case of GMP and GDPβS it is about 100 times lower than that of DNQX, a powerful kainate antagonist) correlates well with their ability to displace KA from retinal KA receptors.

【 授权许可】

Unknown