Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line

FEBS Letters

Yagi, Kunio¹  Ohishi, Nobuko²  Akao, Yukihiro¹  Mizoguchi, Hiromi¹ 

[1] Gifu International Institute of Biotechnology, Yagi Memorial Park, Mitake, Gifu 505-0116, Japan;Institute of Applied Biochemistry, Yagi Memorial Park, Mitake, Gifu 505-0116, Japan

关键词: RCK gene;    rck/p54;    DEAD box/RNA helicase;    Gene transfection;    Growth inhibition;    Guinea pig cell;    FBS;    fetal bovine serum;    PBS;    phosphate-buffered saline;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(98)00605-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We transfected cells of a guinea pig cell line with RCK cDNA inserted in a pIRES1neo expression vector. The overexpression of rck/p54 was confirmed by Western blot and RT-PCR analysis. In two clones expressing rck/p54, the cell growth was highly inhibited; and their anchorage-independent growth, which is an important character of malignant transformation, was not found. These findings are the first evidence that the overexpression of a DEAD box protein/RNA helicase could inhibit substantially cell growth at the translational level.

【 授权许可】

Unknown